The microphthalmia-associated transcription factor (Mitf) gene and its role in regulating eye function

In vivo role of alternative splicing and serine phosphorylation of the microphthalmia-associated transcription factor MITF

*Mammalian Development Section, NINDS, NIH, Bethesda, MD 20892-3706, USA §Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH, Bethesda, MD 20892, USA **Genetics Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA §§NINDS Flow Cytometry Core Facility, NIH, Bethesda, MD 20892, USA Transgenic Core Facility, National Institute of Mental Health, National Institutes...

Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression

N-acetyltransferase 10 (NAT10) has been considered a target for the treatment of human diseases such as cancer and laminopathies; however, its functional role in the biology of melanocytes is questionable. Using a small molecule or small interfering RNA targeting NAT10, we examined the effect of NAT10 inhibition on melanogenesis and melanoma growth in human and mouse melanoma cells. Genetic sil...

Characterizing the Coding Region Determinant-Binding Protein (CRD-BP)-Microphthalmia-associated Transcription Factor (MITF) mRNA interaction

Coding region determinant-binding protein (CRD-BP) binds to the 3'-UTR of microphthalmia-associated transcription factor (MITF) mRNA to prevent its targeted degradation by miR-340. Here, we aim to further understand the molecular interaction between CRD-BP and MITF RNA. Using point mutation in the GXXG motif of each KH domains, we showed that all four KH domains of CRD-BP are important for thei...

The Microphthalmia-associated Transcription Factor (mitf) Requires Swi/snf Enzymes to Activate Melanocyte Specific Genes

Alternative promoter use in eye development: the complex role and regulation of the transcription factor MITF.

During vertebrate eye development, the transcription factor MITF plays central roles in neuroepithelial domain specification and differentiation of the retinal pigment epithelium. MITF is not a single protein but represents a family of isoforms generated from a common gene by alternative promoter/exon use. To address the question of the role and regulation of these isoforms, we first determined...